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Registro Completo |
Biblioteca(s): |
Embrapa Florestas. |
Data corrente: |
05/01/2016 |
Data da última atualização: |
04/02/2016 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
CUNICO, M. M.; TREBIEN, H. A.; GALETTI, F. C.; MIGUEL, O. G.; MIGUEL, M. D.; AUER, C. G.; SILVA, C. L.; SOUZA, A. O. de. |
Afiliação: |
MIRIAM M. CUNICO, UFPR; HERBERT A. TREBIEN, UFPR; FÁBIO C. GALETTI, USP; OBDULIO G. MIGUEL, UFPR; MARILIS D. MIGUEL, UFPR; CELSO GARCIA AUER, CNPF; CÉLIO L. SILVA, USP; ANA OLÍVIA DE SOUZA, Instituto Butantan. |
Título: |
Investigation of local anesthetic and antimycobacterial activity of Ottonia martiana Miq. (Piperaceae). |
Ano de publicação: |
2015 |
Fonte/Imprenta: |
Anais da Academia Brasileira de Ciências, v. 87, n. 4, p. 1991-2000, 2015. |
DOI: |
http://dx.doi.org/10.1590/0001-3765201520140090 |
Idioma: |
Inglês |
Conteúdo: |
Ottonia martiana is a plant popularly known in Brazil by the use for toothache. Ethanolic extract (EE), hexane fraction (HF), dichloromethane fraction (DF) and piperovatine obtained from O. martiana were assayed in vitro and in vivo. The acute toxicity of EE was determined, and LD50 values of 164.5 and 65.0 mg/ kg by the oral and intraperitoneal routes, respectively, indicated a high toxicity for EE in vivo, explaining its popular use by topical administration only. A local anesthetic-like effect of EE and its fractions was observed in experimental models using pain induction, and such effect involved an analgesic action. The antimycobacterial activity of EE, HF, DF and piperovatine was evaluated against Mycobacterium tuberculosis H37Rv ATCC 27924. EE, HF, DF, and piperovatine showed a potential antimycobacterial effect with MICs of 16.0, 62.0, 62.0 and 8.0 ?g/mL, respectively. Piperovatine was more effective than the EE or the other fractions. The selectivity index (SI=IC50/MIC) values calculated for EE, HF, DF and piperovatine based on the MICs and the cytotoxicity against J774 macrophages (IC50 by MTT assay) revealed values of 6.43, 2.34, 1.5 and 9.66, respectively. |
Palavras-Chave: |
Analgesic; Analgésico; Anesthetic; Antimicobacterial; Antimycobacterial; Ottonia martiana; Piperovatina; Piperovatine; Toxicidade. |
Thesagro: |
Anestésico; Espécie Nativa. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/136578/1/2015-Celso-AABC-Investigation.pdf
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Marc: |
LEADER 02219naa a2200349 a 4500 001 2032877 005 2016-02-04 008 2015 bl uuuu u00u1 u #d 024 7 $ahttp://dx.doi.org/10.1590/0001-3765201520140090$2DOI 100 1 $aCUNICO, M. M. 245 $aInvestigation of local anesthetic and antimycobacterial activity of Ottonia martiana Miq. (Piperaceae).$h[electronic resource] 260 $c2015 520 $aOttonia martiana is a plant popularly known in Brazil by the use for toothache. Ethanolic extract (EE), hexane fraction (HF), dichloromethane fraction (DF) and piperovatine obtained from O. martiana were assayed in vitro and in vivo. The acute toxicity of EE was determined, and LD50 values of 164.5 and 65.0 mg/ kg by the oral and intraperitoneal routes, respectively, indicated a high toxicity for EE in vivo, explaining its popular use by topical administration only. A local anesthetic-like effect of EE and its fractions was observed in experimental models using pain induction, and such effect involved an analgesic action. The antimycobacterial activity of EE, HF, DF and piperovatine was evaluated against Mycobacterium tuberculosis H37Rv ATCC 27924. EE, HF, DF, and piperovatine showed a potential antimycobacterial effect with MICs of 16.0, 62.0, 62.0 and 8.0 ?g/mL, respectively. Piperovatine was more effective than the EE or the other fractions. The selectivity index (SI=IC50/MIC) values calculated for EE, HF, DF and piperovatine based on the MICs and the cytotoxicity against J774 macrophages (IC50 by MTT assay) revealed values of 6.43, 2.34, 1.5 and 9.66, respectively. 650 $aAnestésico 650 $aEspécie Nativa 653 $aAnalgesic 653 $aAnalgésico 653 $aAnesthetic 653 $aAntimicobacterial 653 $aAntimycobacterial 653 $aOttonia martiana 653 $aPiperovatina 653 $aPiperovatine 653 $aToxicidade 700 1 $aTREBIEN, H. A. 700 1 $aGALETTI, F. C. 700 1 $aMIGUEL, O. G. 700 1 $aMIGUEL, M. D. 700 1 $aAUER, C. G. 700 1 $aSILVA, C. L. 700 1 $aSOUZA, A. O. de 773 $tAnais da Academia Brasileira de Ciências$gv. 87, n. 4, p. 1991-2000, 2015.
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Registro original: |
Embrapa Florestas (CNPF) |
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Registro Completo
Biblioteca(s): |
Embrapa Soja. |
Data corrente: |
24/03/2021 |
Data da última atualização: |
03/05/2021 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
ALEKCEVETCH, J. C.; PASSIANOTTO, A. L. de L.; FERREIRA, E. G. C.; SANTOS, A. B. dos; SILVA, D. C. G. da; DIAS, W. P.; BELZILE, F.; ABDELNOOR, R. V.; MARCELINO-GUIMARÃES, F. C. |
Afiliação: |
JEAN CARLOS ALEKCEVETCH, Universidade Estadual de Londrina, UEL, Londrina, PR.; ANDRÉ LUIZ DE LIMA PASSIANOTTO, University of Guelph, Guelph, Canada.; EVERTON GERALDO CAPOTE FERREIRA, Universidade Estadual de Londrina, UEL, Londrina, PR.; ADRIANA BROMBINI DOS SANTOS, CNPSO; DANIELLE CRISTINA GREGORIO DA SILVA, CNPSO; WALDIR PEREIRA DIAS, CNPSO; FRANÇOIS BELZILE, Université Laval, Quebec City, Quebec , Canada.; RICARDO VILELA ABDELNOOR, CNPSO; FRANCISMAR CORREA MARCELINO GUIMARA, CNPSO. |
Título: |
Genome-wide association study for resistance to the Meloidogyne javanica causing root-knot nematode in soybean. |
Ano de publicação: |
2021 |
Fonte/Imprenta: |
Theoretical and Applied Genetics, v. 134, p. 777-792, 2021. |
DOI: |
https://doi.org/10.1007/s00122-020-03723-9 |
Idioma: |
Inglês |
Notas: |
Artigo de acesso aberto. |
Conteúdo: |
Key message A locus on chromosome 13, containing multiple TIR-NB-LRR genes and SNPs associated with M. javanica resistance, was identified using a combination of GWAS, resequencing, genetic mapping and expression profiling. Abstract Meloidogyne javanica, a root-knot nematode, is an important problem in soybean-growing areas, leading to severe yield losses. Some accessions have been identified carrying resistance loci to this nematode. In this study, a set of 317 soybean accessions was characterized for resistance to M. javanica. A genome-wide association study was performed using SNPs from genotyping-by-sequencing, and a region of 29.2 kb on chromosome 13 was identified. An analysis of haplotypes showed that SNPs were able to discriminate between susceptible and resistant accessions, with 25 accessions sharing the haplotype associated with resistance. Furthermore, five accessions that exhibited resistance without carrying this haplotype may carry different loci conferring resistance to M. javanica. We also conducted the screening of the SNPs in the USDA soybean germplasm, revealing that several soybean accessions previously reported as resistant to other nematodes also shared the resistance haplotype on chromosome 13. Two SNP-based TaqMan® assays were developed and validated in two panels of soybean cultivars and in biparental populations. In silico analysis of the region associated with resistance identified the occurrence of genes with structural similarity with classical major resistance genes (NBS-LRR genes). Specifically, several nonsynonymous SNPs were observed in Glyma.13g194800 and Glyma.13g194900. The expression profile of these candidate genes demonstrated that the two gene models were up-regulated in the resistance source PI 505,099 after nematode infection. Overall, the SNPs associated with resistance and the genes identified constitute an important tool for introgression of resistance to the root-knot nematode by marker-assisted selection in soybean breeding programs. MenosKey message A locus on chromosome 13, containing multiple TIR-NB-LRR genes and SNPs associated with M. javanica resistance, was identified using a combination of GWAS, resequencing, genetic mapping and expression profiling. Abstract Meloidogyne javanica, a root-knot nematode, is an important problem in soybean-growing areas, leading to severe yield losses. Some accessions have been identified carrying resistance loci to this nematode. In this study, a set of 317 soybean accessions was characterized for resistance to M. javanica. A genome-wide association study was performed using SNPs from genotyping-by-sequencing, and a region of 29.2 kb on chromosome 13 was identified. An analysis of haplotypes showed that SNPs were able to discriminate between susceptible and resistant accessions, with 25 accessions sharing the haplotype associated with resistance. Furthermore, five accessions that exhibited resistance without carrying this haplotype may carry different loci conferring resistance to M. javanica. We also conducted the screening of the SNPs in the USDA soybean germplasm, revealing that several soybean accessions previously reported as resistant to other nematodes also shared the resistance haplotype on chromosome 13. Two SNP-based TaqMan® assays were developed and validated in two panels of soybean cultivars and in biparental populations. In silico analysis of the region associated with resistance identified the occurrence of genes with structural similarity with classical ... Mostrar Tudo |
Thesagro: |
Galha; Genoma; Meloidogyne Javanica; Nematóide; Resistência; Soja. |
Thesaurus NAL: |
Genome-wide association study; Resistance mechanisms; Root-knot nematodes; Soybeans. |
Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/222104/1/Alekcevetch2021-Article-Genome-wideAssociationStudyFor.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/222143/1/Alekcevetch2021-Article-Genome-wideAssociationStudyFor.pdf
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Marc: |
LEADER 03125naa a2200361 a 4500 001 2130887 005 2021-05-03 008 2021 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1007/s00122-020-03723-9$2DOI 100 1 $aALEKCEVETCH, J. C. 245 $aGenome-wide association study for resistance to the Meloidogyne javanica causing root-knot nematode in soybean.$h[electronic resource] 260 $c2021 500 $aArtigo de acesso aberto. 520 $aKey message A locus on chromosome 13, containing multiple TIR-NB-LRR genes and SNPs associated with M. javanica resistance, was identified using a combination of GWAS, resequencing, genetic mapping and expression profiling. Abstract Meloidogyne javanica, a root-knot nematode, is an important problem in soybean-growing areas, leading to severe yield losses. Some accessions have been identified carrying resistance loci to this nematode. In this study, a set of 317 soybean accessions was characterized for resistance to M. javanica. A genome-wide association study was performed using SNPs from genotyping-by-sequencing, and a region of 29.2 kb on chromosome 13 was identified. An analysis of haplotypes showed that SNPs were able to discriminate between susceptible and resistant accessions, with 25 accessions sharing the haplotype associated with resistance. Furthermore, five accessions that exhibited resistance without carrying this haplotype may carry different loci conferring resistance to M. javanica. We also conducted the screening of the SNPs in the USDA soybean germplasm, revealing that several soybean accessions previously reported as resistant to other nematodes also shared the resistance haplotype on chromosome 13. Two SNP-based TaqMan® assays were developed and validated in two panels of soybean cultivars and in biparental populations. In silico analysis of the region associated with resistance identified the occurrence of genes with structural similarity with classical major resistance genes (NBS-LRR genes). Specifically, several nonsynonymous SNPs were observed in Glyma.13g194800 and Glyma.13g194900. The expression profile of these candidate genes demonstrated that the two gene models were up-regulated in the resistance source PI 505,099 after nematode infection. Overall, the SNPs associated with resistance and the genes identified constitute an important tool for introgression of resistance to the root-knot nematode by marker-assisted selection in soybean breeding programs. 650 $aGenome-wide association study 650 $aResistance mechanisms 650 $aRoot-knot nematodes 650 $aSoybeans 650 $aGalha 650 $aGenoma 650 $aMeloidogyne Javanica 650 $aNematóide 650 $aResistência 650 $aSoja 700 1 $aPASSIANOTTO, A. L. de L. 700 1 $aFERREIRA, E. G. C. 700 1 $aSANTOS, A. B. dos 700 1 $aSILVA, D. C. G. da 700 1 $aDIAS, W. P. 700 1 $aBELZILE, F. 700 1 $aABDELNOOR, R. V. 700 1 $aMARCELINO-GUIMARÃES, F. C. 773 $tTheoretical and Applied Genetics$gv. 134, p. 777-792, 2021.
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